RNA Therapeutics Function Design and Delivery by Mouldy Sioud , Humana

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Humana RNA Therapeutics Function Design and Delivery by Mouldy Sioud

Central to the synthesis of proteins, the performance of catalysis, and many other physiological processes, the aberrant expression of which can be linked to human diseases including cancers, RNA has proven to be key target for therapeutics as well as a tool for therapy. In RNA Therapeutics: Function, Design, and Delivery, expert contributors from a broad spectrum of scientific backgrounds highlight the roles that messenger RNAs and small RNAs can play in biology and medicine. While covering the five major RNA-based drugs, namely the use of ribozymes to cleave and/or correct mRNA transcript, the use of siRNA for targeted silencing of gene transcripts, the use of aptamers, like short RNA molecules, for neutralizing the protein functions, the use mRNA-transfected DCs to activate immune system against tumor cells, as well as the use of RNA to reprogram T and/or DC cell function, this extensive volume brings together the fields of coding (mRNA) and non-coding RNA such as ribozymes, RNAse P, siRNAs, and miRNAs into one convenient source. Written in the highly successful Methods in Molecular Biology (TM) series format, the cutting-edge protocol chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and practical tips on troubleshooting and avoiding known pitfalls. Also, the book contains several excellent reviews for teaching purposes._x000D__x000D__x000D_Authoritative and comprehensive, RNA Therapeutics: Function, Design, and Delivery provides key models and tools which will assist researchers in increasing our understanding of RNA functions, modifications, and their involvement in diseases in order to lead to the design of vital new RNA-based therapeutics._x000D_ Table of contents : - _x000D_ 1. RNA Modifications: A Mechanism that Modulates Gene Expression_x000D_ John Karijolich, Athena Kantartzis, and Yi-Tao Yu_x000D_ _x000D_ 2. Quantitative Analysis of RNA Modifications_x000D_ John Karijolich, Athena Kantartzis, and Yi-Tao Yu_x000D_ _x000D_ 3. Advances in RNA Sensing by the Immune System: Separation of siRNA Unwanted Effects from RNA Interference_x000D_ Mouldy Sioud_x000D_ _x000D_ 4. Progress in siRNA Delivery: A Multifunctional Approach by Polymeric Nanoparticles_x000D_ Weiwei Gao, Zeyu Xiao, Alex Radovic-Moreno, Jinjun Shi, Robert Langer, and Omid C. Farokhzad_x000D_ _x000D_ 5. Optimized Protocols for siRNA Delivery into Monocytes and Dendritic Cells_x000D_ Anne Mobergslien and Mouldy Sioud_x000D_ _x000D_ 6. Systemic Delivery of Synthetic siRNAs_x000D_ Dag R. Sorensen and Mouldy Sioud_x000D_ _x000D_ 7. What Are the Key Targeted Delivery Technologies of siRNA Now?_x000D_ Mouldy Sioud_x000D_ _x000D_ 8. Using OligoWalk to Identify Efficient siRNA Sequences_x000D_ David H. Mathews_x000D_ _x000D_ 9. Jet-Injection of Short Hairpin RNA Encoding Vectors into Tumor Cells_x000D_ Wolfgang Walther, Ulrike Stein, and Hermann Lage_x000D_ _x000D_ 10. Short Hairpin RNA (shRNA): Design, Delivery, and Assessment of Gene Knockdown_x000D_ Chris B. Moore, Elizabeth H. Guthrie, Max Tze-Han Huang, and Debra Taxman_x000D_ _x000D_ 11. Effective Pol III-Expressed Long Hairpin RNAs Targeted to Multiple Unique Sites of HIV-1_x000D_ Sheena M. Saayman, Patrick Arbuthnot, and Marc S. Weinberg_x000D_ _x000D_ 12. Functional Studies on RNA-Transfected Cell Microarrays_x000D_ Christina Saeten Fjeldbo, Kristine Misund, Clara-Cecilie Gunther, Mette Langaas, Tonje Strommen Steigedal, Liv Thommesen, Astrid Laegreid, and Torunn Bruland_x000D_ _x000D_ 13. Transfection Microarrays for High-Throughput Phenotypic Screening of Genes Involved in Cell Migration_x000D_ Reiko Onuki-Nagasaki, Akira Nagasaki, Kazumi Hakamada, Taro Q.P. Uyeda, Satoshi Fujita, Masato Miyake, and JunMiyake_x000D_ _x000D_ 14. Intron-Mediated RNA Interference, Intronic MicroRNAs, and Applications_x000D_ Shao-Yao Ying, Chen Pu Chang, and Shi-Lung Lin_x000D_ _x000D_ 15. Inhibition of the microRNA Pathway in Zebrafish by siRNA_x000D_ Anders Fjose and Xiao-Feng Zhao_x000D_ _x000D_ 16. Profiling of miRNA Expression and Prediction of Target Genes_x000D_ Mouldy Sioud_x000D_ _x000D_ 17. Small RNA Cloning_x000D_ Seungil Ro and Wei Yan_x000D_ _x000D_ 18. In situ Hybridization Detection of microRNAs_x000D_ Rui Song, Seungil Ro, and Wei Yan_x000D_ _x000D_ 19. Detection and Quantitative Analysis of Small RNAs by PCR_x000D_ Seungil Ro and Wei Yan_x000D_ _x000D_ 20. In Vivo Reprogramming of Human Telomerase Reverse Transcriptase (hTERT) by Trans-Splicing Ribozyme to Target Tumor Cells_x000D_ Sang-Jin Lee, Seong-Wook Lee, Jin-Sook Jeong, and In-Hoo Kim_x000D_ _x000D_ 21. Design and Function of Triplex Hairpin Ribozymes_x000D_ Guillermo Aquino-Jarquin, Ramiro Rojas-Hernandez, and Luis Marat Alvarez-Salas_x000D_ _x000D_ 22. Catalytic M1GS RNA as an Antiviral Agent in Animals_x000D_ Yong Bai, Paul Jay Fannin Rider, and Fenyong Liu_x000D_ _x000D_ 23. Using Live Cells to Generate Aptamers for Cancer Study_x000D_ Ling Meng, Kwame Sefah, Dalia Lopez Colon, Hui Chen, Meghan O'Donoghue, Xiangling Xiong, and Weihong Tan_x000D_ _x000D_ 24. Primer-Free Aptamer Selection Using a Random DNA Library_x000D_ Weihua Pan and Gary A. Clawson_x000D_ _x000D_ 25. Development of TLR 7/8 Small RNA Antagonists_x000D_ Mouldy Sioud_x000D_ _x000D_ 26. Modulation of Dendritic Cell Maturation and Function by siRNA-Bearing 5'-Triphosphate_x000D_ Mouldy Sioud_x000D_ _x000D_ 27. Immunotherapy of Cancer with Dendritic Cells Loaded with Tumor Antigens and Activated through mRNA Electroporation_x000D_ An M.T. Van Nuffel, Jurgen Corthals, Bart Neyns, Carlo Heirman, Kris Thielemans, and Aude Bonehill_x000D_ _x000D_ 28. Non-MHC-Dependent Redirected T Cells Against Tumor Cells_x000D_ Hilde Almasbak, Marianne_x000D_