Description
Humana Receptor Binding Techniques 2Nd Editon by Anthony P. Davenport
A comprehensive collection of readily reproducible methods for studying receptors in silico, in vitro, and in vivo. These cutting-edge techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knockout mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan PCR. These book equips the researcher with techniques for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified. Receptor Databases and Computational Websites for Ligand BindingBrinda K. Rana and Paul A. InselIdentification of Orphan G Protein-Coupled Receptor Ligands Using FLIPR (R) AssaysNicola M. Robas and Mark D. FidockQuantitative Analysis of Orphan G Protein-Coupled Receptor mRNAs by TaqMan (R) Real-Time PCR: G2A and GPR4 Lysophospholipid Receptor Expression in Leukocytes and in a Rat Myocardial Infarction-Heart Failure ModelStephen A. Douglas, Zhaohui Ao, Douglas G. Johns, Kristeen Maniscalco, Robert N. Willette, Lea Sarov-Blat, John P. Cogswell, Sheila Seepersaud, Paul Murdock, Klaudia M. Steplewski, and Lisa PatelmRNA: Detection by In Situ and Northern HybridizationAlessandra P. Princivalle, Rachel M. C. Parker, Terri J. Dover, and Nicholas M. BarnesRadioligand-Binding and Molecular-Imaging Techniques for the Quantitative Analysis of Established and Emerging Orphan Receptor SystemsAnthony P. Davenport and Rhoda E. KucMeasurement of Radioligand Binding by Scintillation Proximity AssayJenny Berry and Molly Price-JonesAutoradiography of Enzymes, Second Messenger Systems, and Ion ChannelsDavid A. Walsh and John WhartonImmunocytochemical Localization of Receptors Using Light and Confocal Microscopy With Application to the Phenotypic Characterization of Knock-Out MiceAnthony P. Davenport and Rhoda E. KucLive Cell Imaging of G Protein-Coupled ReceptorsBurkhard Wiesner, Michael Beyermann, and Alexander OkscheImaging and Characterization of Radioligands for Positron Emission Tomography Using Quantitative Phosphor Imaging AutoradiographyPeter Johnstroem and Anthony P. DavenportDynamic In Vivo Imaging of Receptors in Small Animals Using Positron Emission TomographyPeter Johnstroem, Tim D. Fryer, Hugh K. Richards, Olivier Barret, and Anthony P. DavenportIndex