Description
HUMANA PRESS Reporter Genes A Practical Guide (Methods In Molecular Biology) 2007 Edition by DON ANSON
Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. This book describes practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available. Preface. Donald S. Anson.1. Methodologies for staining and visualisation of '-galactosidase inembryos and tissues. Siobhan Loughna* and Deborah Henderson.2. Immunohistochemical Detection of Beta-Galactosidase or GreenFluorescence Protein on Tissue Sections. Philip A. Seymour and MaikeSander*.3. Detection of Reporter Gene Expression in Murine Airways. MariaLimberis*, Peter Bell, and James M. Wilson.4. Three dimensional analysis of molecular signals with episcopicimaging techniques. Wolfgang J. Weninger* & Timothy J. Mohun5. Fluorescent proteins for cell biology. George H. Patterson*6. Detection of GFP during nervous system development in Drosophilamelanogaster. Karin Edoff, James S. Dods and Andrea H. Brand*7. Autofluorescent Proteins for Flow Cytometry. Charles G. Bailey andJohn E. J. Rasko*8. Fluorescent protein reporter systems for single cell measurements.Steven K. Dower*, Eva E. Qwarnstrom and Endre Kiss-Toth9. Subcellular Imaging of cancer cells in live mice. Robert M. Hoffman*10. Non-invasive Imaging of Molecular Events with BioluminescentReporter Genes in Living Subjects. Pritha Ray and Sanjiv Sam Gambhir*.11. Green Fluorescent Protein as a Tracer in Chimeric Tissues. HaraldJockusch* and Daniel Eberhard