Single Cell Diagnostics Methods And Protocols by Alan R. Thornhill , Humana Press Inc.

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Humana Press Inc. Single Cell Diagnostics Methods And Protocols by Alan R. Thornhill

This book applies modern molecular diagnostic techniques to the analysis of single cells, small numbers of cells, or cell extracts. Emphasis is placed on non-invasive analysis of single cell metabolites and the direct analysis of RNA and DNA from single cells, with a focus on polymerase chain reaction and fluorescence in situ hybridization. In particular, this handbook is essential for practitioners providing care for couples seeking treatment for infertility._x000D_ _x000D_ Table of Contents_x000D_ _x000D_ 1. Non-invasive metabolic assessment of single cells_x000D_ David K. Gardner _x000D_ _x000D_ 2. Laser Capture Microdissection for Analysis of Single Cells _x000D_ Nobuki Nakamura, Katharina Ruebel, Long Jin, Xiang Qian, Heyu Zhang, and Ricardo V. Lloyd_x000D_ _x000D_ 3. Fluorescence in situ hybridization (FISH) on Single Cells (Sex Determination and Chromosome Rearrangements)_x000D_ Paul N. Scriven and Caroline Mackie Ogilvie_x000D_ _x000D_ 4. Single cell PCR for PGD: methods, strategies and limitations_x000D_ Karen Sermon and Martine De Rycke_x000D_ _x000D_ 5. Real-Time Quantitative PCR Measurement of Male Fetal DNA in maternal plasma_x000D_ Bernhard Zimmermann, Ahmad El-Sheikhah, Kypros Nicolaides, Wolfgang Holzgreve, Sinuhe Hahn_x000D_ _x000D_ 6. Cell-Free Fetal DNA Plasma Extraction and Real Time-PCR Quantification_x000D_ Jill L. Maron, Kirby L. Johnson, and Diana W. Bianchi_x000D_ _x000D_ 7. LATE-PCR and allied technologies: Real-time detection strategies for rapid, reliable diagnosis from single cells_x000D_ Kenneth E. Pierce and Lawrence J. Wangh_x000D_ 8. Efficient isothermal amplification of the entire genome from single cells _x000D_ Karen V. Schowalter, Jolene R. Fredrickson and Alan R Thornhill_x000D_ _x000D_ 9. Comparative Genomic Hybridization on single cells_x000D_ Lucille Voullaire and Leeanda Wilton _x000D_ _x000D_ 10. Generation of amplified RNAs and cDNA libraries from single mammalian cells_x000D_ James Adjaye_x000D_ _x000D_ 11. Real-time PCR to measure gene expression in single cells_x000D_ Dagan Wells_x000D_ 12. Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative Genomic Hybridisation (Array CGH) _x000D_ Dong Gui Hui, Xin Yuan Guan, and Nicole Hussey_x000D_ _x000D_ 13. Microarray technology for mutation analysis of low template DNA samples_x000D_ Chelsea Salvado and David Cram_x000D_