Stem Cell Assays by Mohan C. Vemuri , Humana Press Inc.

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Humana Press Inc. Stem Cell Assays by Mohan C. Vemuri

The scope for improving health care using stem cell therapies is thrilling, but has considerable technical challenges and methodological constraints that need to be addressed. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, this book presents scientific advances in stem cell methods for a wider use by novice and expert scientists, through the series of Methods in Molecular Biology._x000D_ _x000D_ Book Title: Stem Cell Assays _x000D_ Publisher: Humana Press_x000D_ Editor : Mohan C. Vemuri Series_x000D_ Editor : John Walker_x000D_ Series : Methods in Molecular Biology Date of_x000D_ Release: Late 2006 / Early 2007_x000D_ _x000D_ _x000D_ Contents_x000D_ _x000D_ Preface_x000D_ Contributors_x000D_ Guide to the companion CD_x000D_ _x000D_ _x000D_ 1 Derivation of Human Embryonic Stem Cells in xeno-free conditions_x000D_ Mohan C. Vemuri, Tim Schimmel, Pere Colls, Santiago Munne and Jacques_x000D_ Cohen_x000D_ _x000D_ _x000D_ 2 Feeder Layer Free Culture System For Human Embryonic Stem Cells_x000D_ Michal Amit_x000D_ _x000D_ _x000D_ 3 Digital Imaging of Stem Cells by Electron Microscopy_x000D_ Henry Sathananthan and Stefania Nottola_x000D_ _x000D_ _x000D_ 4 A Controlled Cooling Protocol For Cryopreservation Of Human And_x000D_ Non-Human Primate Embryonic Stem Cells_x000D_ Carol B. Ware and Szczepan Baran_x000D_ _x000D_ _x000D_ 5 Cell Surface Markers In Human Embryonic Stem Cells_x000D_ Raj R. Rao, Alison Venable Johnson and Steven L. Stice_x000D_ _x000D_ _x000D_ 6 Generation Of Monoclonal Antibody Library Against Human Embryonic_x000D_ Stem Cells_x000D_ Micha Drukker, Christina Muscat, Irving L. Weissman_x000D_ _x000D_ _x000D_ 7 Analytical Methods for Cancer Stem Cells - Review chapter_x000D_ Vinagolu K. Rajasekhar_x000D_ _x000D_ _x000D_ 8 Micro RNA Profiling: An Easy and Rapid Method to Screen and_x000D_ Characterize Stem Cell Populations_x000D_ Uma Lakshmipathy, Bradley Love, Christopher Adams, Bhaskar_x000D_ Thyagarajan, and_x000D_ Jonathan D. Chesnut_x000D_ _x000D_ _x000D_ 9 Gene Transfer Via Nucleofection into Adult And Embryonic Stem Cells_x000D_ Uma Lakshmipathy, Shannon Buckley and Catherine Verfaillie_x000D_ _x000D_ _x000D_ 10 RNAi-Knockdown Of Transcription Factor Pu.1 In The Differentiation_x000D_ Of Mouse Embryonic_x000D_ Stem Cells_x000D_ Gang-Ming Zou, Meredith A. Thompson and Mervin C. Yoder_x000D_ _x000D_ _x000D_ 11 StemBase - A Resource for the Analysis of Stem Cell Gene Expression_x000D_ Data - Review chapter_x000D_ Christopher J. Porter, Gareth A. Palidwor, Reatha Sandie, Paul M._x000D_ Krzyzanowski, Enrique M. Muro, Carolina Perez-Iratxeta, Miguel A._x000D_ Andrade-Navarro_x000D_ _x000D_ _x000D_ 12 Isolation Of Stem Cells From Human Umbilical Cord Blood_x000D_ Nishant P. Reddy, Mohan C. Vemuri and Reddanna Pallu_x000D_ _x000D_ _x000D_ 13 Ex Vivo Expansion Of Hematopoietic Stem Cells From Human Cord Blood_x000D_ In Serum-Free Conditions_x000D_ Chao-Ling Yao and Shiaw-Min Hwang_x000D_ _x000D_ _x000D_ 14 Hematopoietic Colony Forming Cell Assays_x000D_ Carla Pereira, Emer Clarke and Jackie Damen_x000D_ _x000D_ _x000D_ 15 Assays For Alloreactive Responses By PCR_x000D_ Patrick Stordeur_x000D_ _x000D_ _x000D_ 16 Immune Properties of Mesenchymal Stem Cells Review chapter_x000D_ Panagiota A. Sotiropoulou and Michael Papamichail_x000D_ _x000D_ _x000D_ 17 Clinical Grade Expansion of Human Bone Marrow Mesenchymal Stem Cells_x000D_ Panagiota A. Sotiropoulou, Sonia A. Perez and Michael Papamichail_x000D_ _x000D_ _x000D_ 18 Adenoviral Transduction of Mesenchymal Stem Cells_x000D_ Pablo Bosch and Steven L. Stice_x000D_ _x000D_ _x000D_ 19 Directed Differentiation of Human Embryonic Stem Cells to Dendritic_x000D_ Cells_x000D_ Maxim A. Vodyanik and Igor I. Slukvin_x000D_ _x000D_ _x000D_ 20 Insulin Producing Cells From Embryonic Stem Cells: Experimental_x000D_ Considerations_x000D_ Enrique Roche, Roberto Ensenat-Waser, Nestor Vicente-Sala, Alfredo_x000D_ Santana,_x000D_ Martin Zenke and Juan Antonio Rei_x000D_ _x000D_ _x000D_ _x000D_ 21 Efficient Generation of Dopamine Neurons from Human Embryonic Stem_x000D_ (hES) Cells_x000D_ Chang-Hwan Park and Sang-Hun Lee_x000D_ _x000D_ _x000D_ 22 Isolation Of Oligodendroglial Cells From Cultured Neural_x000D_ Stem/Progenitors_x000D_ _x000D_ Frank Zeigler and Stephen G. Hall_x000D_ _x000D_ _x000D_ 23 Differentiation of Human Embryonic Stem Cells towards the_x000D_ Chondrogenic Lineage_x000D_ Wei Seong Toh, Zheng Yang Boon Chin Heng and Tong Cao_x000D_ _x000D_ _x000D_ 24 Cartilage Tissue Engineering: Directed differentiation of embryonic_x000D_ stem cells in Three-Dimensional Hydrogel Culture_x000D_ Nathaniel S. Hwang, Shyni Varghese and Jennifer Elisseeff_x000D_