Description
Humana The Urinary Proteome Methods And Protocols 2010 Edition by Alex J. Rai
This book is intended for scientifc researchers clinical laboratorians clinical and translational scientists and others interested in proteomics and biomarker discovery. Urine is one of the most easily accessible biological samples and it provides a treasure trove of molecules important in clinical diagnostics. In this book we review briefly the classical urine tests that are performed in the clinical laboratory and then delve into the state-of-the-art methods for proteomic analysis using urine specimens. The most recent advances are discussed with regard to sample preparation data analysis and fnally methods and applications. A multitude of examples are provided including procedural details for the identifcation and characterization of urine biomarkers that hold potential for the diagnosis and treatment of many different disease conditions. The text is arranged so as to read systematically: introduction sample preparation methods applications and data analysis. However it does not necessarily require the reader to read it from start to fnish. Each chapter is organized such that it can be read individually without requiring knowledge from other chapters. I would like to thank the many individuals who made this book possible. These include the many authors who contributed to each of the individual chapters the corresponding authors who took responsibility in providing the complete and fnished versions solicited for the peer review process and the many scientifc reviewers who provided their valuable input and guidance. Table of contents : 1. Introduction to Urinalysis: Historical Perspectives and Clinical ApplicationGerman Echeverry Glen L. Hortin and Alex J. Rai2. A Primer on Clinical Applications and Assays Using Urine: Focus on Analysis of Plasma Cell Dyscrasias Using Automated Electrophoresis and ImmunofixationBrianne Olivieri and Alex J. Rai3. Application of Free Flow Electrophoresis to the Analysis of the Urine ProteomeAude L. Foucher David R. Craft and Craig A. Gelfand4. Standardized Pre-Processing of Urine for Proteome AnalysisGeorg Martin Fiedler Uta Ceglarek Alexander Leichtle and Joachim Thiery5. Different Sample Preparation and Detection Methods for Normal and Lung Cancer Urinary Proteome AnalysisSupachok Sinchaikul Payungsak Tantipaiboonwong Supawadee Sriyam Ching Tzao Suree Phutrakul and Shui-Tein Chen6. Isolation and Purification of Exosomes in UrinePatricia A. Gonzales Hua Zhou Trairak Pisitkun Nam Sun Wang Robert A. Star Mark A. Knepper and Peter S.T. Yuen7. Bioinformatics of the Urinary ProteomeLaurence D. Parnell and Christine M.E. Schueller8. PROTIS: Use of Combined Biomarkers for Providing Diagnostic Information on Disease StatesWalter Hofmann Cornelia Sedlmeir-Hofmann Miroslav Ivandic Dagmar Ruth and Peter Luppa9. Statistical Contributions to Proteomic ResearchJeffrey S. Morris Keith A. Baggerly Howard B. Gutstein and Kevin R. Coombes10. A Review of Experimental Design Best Practices for Proteomics Based Biomarker Discovery: Focus on SELDI-TOFRebecca E. Caffrey11. Urine Proteomic Profiling for Biomarkers of Acute Renal Transplant RejectionShu-Ling Liang and William Clarke12. Surface Plasmon Resonance Biosensorics in Urine ProteomicsPeter B. Luppa Jochen Metzger and HeikeSchneider13. Urinary Proteins for the Diagnosis of Obstructive Sleep Apnea SyndromeAyelet Snow David Gozal Roland Valdes Jr. and Saeed A. Jortani14. Immune Response Biomarker Profiling Application on ProtoArray (R) Protein MicroarraysBarry Schweitzer Lihao Meng Dawn Mattoon and Alex J. Rai15. Design and Validation of an Immunoaffinity LC-MS/MS Assay for the Quantification of a Collagen Type II Neoepitope Peptide in Human Urine: Application as a Biomarker of OsteoarthritisOlga Nemirovskiy Wenlin (Wendy) Li and Gabriella Szekely-Klepser16. Cell Specific Biomarkers in Renal Medicine and ResearchMartin Shaw17. Proteomic Assays for the Detection of Urothelial CancerKris E. Gaston and H. Barton Grossman18. Urine Proteomic Analysis: Use of Two-Dimensional Gel Electrophoresis Isotope Coded Affinity Tags and Capillary ElectrophoresisKimia Sobhani19. Proteomic Analysis of Pancreatic Secretory Trypsin Inhibitor / Tumor-Associated Trypsin Inhibitor from Urine of Patients with Pancreatitis or Prostate CancerLeena Valmu Suvi Ravela and Ulf-Hakan Stenman