Description
HUMANA PRESS Thyroid Hormone Receptors Methods And Protocols (Methods In Molecular Biology Volume 202) 2002 Edition by BANIAHMAD ARIA
A panel of outstanding investigators surveys and explains the major cutting-edge methods used in thryroid receptor (TR) research and explains their practical experimental details. Described in step-by-step detail to ensure robust experimental results, the techniques presented cover a wide variety of key areas, including TR in development and knockout (mouse and Xenopus), transcriptional regulation by TRs in both cell-free systems and in living cells, and TR mutant analysis of patients. Additional methods provide powerful tools for the isolation of TR-regulated protein complexes, for studying the oncogene v-Erba in blood cell differentiation, and for target gene analysis in the brain. Microarray chip methods are also presented for analyzing the organs of transgenic mice to identify target genes in the liver. Introduction to Thyroid Hormone ReceptorsAria BaniahmadNull Mutant Mice for Thyroid Hormone ReceptorsKarine Gauthier, Denise Aubert, Olivier Chassande, Frederic Flamant, and Jacques SamarutTransgenic Targeting of a Dominant Negative Corepressor to Liver and Analyses by cDNA MicroarrayXu Feng, Paul Meltzer, and Paul M. YenThe Role of Thyroid Hormone Receptors in the HeartWolfgang H. Dillmann and Bernd R. GlossAnalysis of Thyroid Hormone-Dependent Genes in the Brain by In Situ HybridizationJuan Bernal and Ana Guadano-Ferraz.The v-erbA Oncogene: Assessing Its Differentiation-Blocking Ability Using Normal Chicken Erythrocytic Progenitor Cells,Olivier GandrillonGene Regulation by Thyroid Hormone ReceptorsHelmut Dotzlaw and Aria BaniahmadMolecular Analysis of Human Resistance to Thyroid Hormone SyndromeSunnie M. Yoh and Martin L. PrivalskyFunction of Thyroid Hormone Receptors During Amphibian DevelopmentSashko Damjanovski, Laurent M. Sachs, and Yun-Bo ShiTranscriptional Regulation by Thyroid Hormone Receptor in ChromatinJiemin WongGene Activation by Thyroid Hormone Receptor In Vitro and Purification of the TRAP Coactivator ComplexJoseph D. FondellIndex