Description
Springer Tissue Culture of Epithelial Cells 1st Editon 2012 Softbound by Mary Taub
Epithelial cells are present in many different tissues in the body, and possess a diverse number of functional properties. However, all epithelial cells share some common characteristics. The cells possess a morphological polarity (an-apical and basolateral surface), and are interconnected by tight junctions. The epithelial cells also possess the capacity to transport select solutes across the monolayer. Transport systems localized on either the apical or basolateral surface are respon sible for this vectorial transport. Such characteristics of epithelial cells can be examined in the tissue culture situation. This volume discusses the use of cell culture techniques to study these fundamental properties of epithelial cells. Ma jor questions concerning epithelia which may be examined in culture are ad dressed. The approaches which are taken to answer these questions are described in detail with regards to kidney cell cultures. Similar investigations may be done with epithelial cell cultures derived from other tissues, following the kidney cell culture paradigm. Section I Domes.- 1 Inducers of Dome Formation in Epithelial Cell Cultures including Agents That Cause Differentiation.- Section II Electrophysiology and Ion Transport in MDCK Cells.- 2 Electrical Properties of MDCK Cells.- 3 Ion Transport in MDCK Cells.- 4 Application of the Microbiological Approach to the Study of Passive Monovalent Salt Transport in a Kidney Epithelial Cell Line, MDCK.- Section III Transport of Neutral Solutes by LLC—PK1 Cells.- 5 Sugar Transport in the Renal Epithelial Cell Culture.- 6 Neutral Amino Acid Transport in Cultured Kidney Tubule Cells.- 7 Transepithelial Transport in Cell Culture: Mechanism and Bioenergetics of Na+, d-Glucose Cotransport.- Section IV Membrane Proteins and Hormone Receptors.- 8 Viruses in the Study of the Polarity of Epithelial Membranes.- 9 Hormone Receptors and Response in Cultured Renal Epithelial Cell Lines.- 10 Monoclonal Antibodies to Integral Membrane Transport and Receptor Proteins: Novel Reagents for Protein Purification.- Section V Epithelial Cell Cultures and Hormonally Defined Medium.- 11 Estrogen-Dependent Kidney Tumors.- 12 Hormonally Defined, Serum-Free Media for Epithelial Cells in Culture.- 13 Importance of Hormonally Defined, Serum-Free Medium for In Vitro Studies Concerning Epithelial Transport.