Transmembrane Signaling Protocols by Hydar Ali, Haribabu Bodduluri , Springer

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Springer Transmembrane Signaling Protocols by Hydar Ali, Haribabu Bodduluri

The previous edition of Transmembrane Signaling Protocols was published in 1998. Since then the human genome has been completely sequenced and new methods have been developed for the use of microarrays and proteomics to analyze global changes in gene expression and protein profiles. These advances have increased our ability to understand transmembrane signaling processes in much greater detail. They have also simultaneously enhanced our ability to determine the role of a large number of newly identified molecules in signaling events. In addition, novel video microscopy methods have been developed to image transmembrane signaling events in live cells in real time. In view of these major advances, it is time to update the previous edition. Because of the success of that volume, we have chosen to keep the essential character of the book intact. Introductory chapters from experts have been included to provide overall perspective and an overview of recent advances in signal transduction pathways. The individual chapters now include comp- hensive detailed methods, studies in genetically tractable systems, fluorescence microscopy in live single cells, ex vivo analysis of primary cells from tra- genic mice, as well as genomic and proteomic approaches to the analysis of transmembrane signaling events. We would like to express our deep gratitude to the coauthors of this publi- tion. We hope that Transmembrane Signaling Protocols, Second Edition will serve as a valuable resource for future progress in the study of signal transd- tion pathways._x000D_ _x000D_Part I. Overviews_x000D_ _x000D_ Transmembrane Signaling by G Protein-Coupled Receptors_x000D_ Louis M. Luttrell_x000D_ _x000D_ Crosstalk Coregulation Mechanisms of G Protein-Coupled Receptors and Receptor Tyrosine Kinases_x000D_ Kanchana Natarajan and Bradford C. Berk_x000D_ _x000D_ Protein-Protein Interactions in Signaling Cascades_x000D_ Bruce J. Mayer_x000D_ _x000D_ Part II. Specific Topics_x000D_ _x000D_ A. Membrane Receptors and Signaling_x000D_ _x000D_ Biological Role of the CXCR4-SDF-1 Axis in Normal Human Hematopoietic Cells_x000D_ Marcin Majka and Mariusz Z. Ratajczak_x000D_ _x000D_ Functional Expression of CXCR4 in Saccharomyces cerevisiae in the Development of Powerful Tools for the Pharmacological Characterization of CXCR4_x000D_ Zi-xuan Wang, James R. Broach, and Stephen C. Peiper_x000D_ _x000D_ Characterization of Constitutively Active Mutants of G Protein-Coupled Receptors_x000D_ Jean-Marc Navenot, Zi-xuan Wang, and Stephen C. Peiper_x000D_ _x000D_ G Protein-Coupled Receptor Dimerization and Signaling_x000D_ Mario Mellado, Antonio Serrano, Carlos Martinez-A, and Jose Miguel Rodriguez-Frade_x000D_ _x000D_ Real-Time Analysis of G Protein-Coupled Receptor Signaling in Live Cell_x000D_ Venkatakrishna R. Jala and Bodduluri Haribabu_x000D_ _x000D_ B. Lipid Raft and Caveolae in Transmembrane Signaling_x000D_ _x000D_ Isolation of Membrane Rafts and Signaling Complexes_x000D_ Kathleen Boesze-Battaglia_x000D_ _x000D_ Methods for the Study of Signaling Molecules in Membrane Lipid Rafts and Caveolae_x000D_ Rennolds S. Ostrom and Paul A. Insel_x000D_ _x000D_ C. Protein-Protein Interaction in Transmembrane Signaling_x000D_ _x000D_ Bioluminescence Resonance Energy Transfer to Monitor Protein-Protein Interactions_x000D_ Tarik Issad and Ralf Jockers_x000D_ _x000D_ Identification of Interacting Proteins Using the Yeast Two-Hybrid Screen_x000D_ Kelly L. Jordan-Sciutto and Marshall B. Montgomery_x000D_ _x000D_ Analysis of PDZ Domain Interactions Using Yeast Two-Hybrid and Coimmunoprecipitation Assays_x000D_ Hyun Woo Lee, Jaewon Ko, and EunjoonKim_x000D_ _x000D_ Clustering Assay for Studying the Interaction of Membrane Proteins With PDZ Domain Proteins_x000D_ Jaewon Ko and Eunjoon Kim_x000D_ _x000D_ D. Rho Guanosine Triphosphatases and Reconstitution of Signaling Complexes_x000D_ _x000D_ Mammalian Cell Microinjection Assay to Study the Function of Rho Family Guanosine Triphosphatases_x000D_ Ritu Garg and Anne J. Ridley_x000D_ _x000D_ Affinity-Based Assay of Rho Guanosine Triphosphatase Activation_x000D_ Mary Stofega, Celine DerMardirossian, and Gary M. Bokoch_x000D_ _x000D_ Assay of Phospholipase D Activity in Cell-Free Systems_x000D_ Shankar S. Iyer and David J. Kusner_x000D_ _x000D_ Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipase D_x000D_ Amanda Fensome-Green and Shamshad Cockcroft_x000D_ _x000D_ E. Genomics and Proteomics_x000D_ _x000D_ Analysis of Global Gene Expression Profiles Activated by Chemoattractant Receptors_x000D_ Fernando O. Martinez and Massimo Locati_x000D_ _x000D_ Genetic Reconstitution of Bone Marrow for the Study of Signal Transduction Ex Vivo_x000D_ Martha S. Jordan_x000D_ _x000D_ Proteomic Analysis of Human Neutrophils_x000D_ George Lominadze, Richard A. Ward, Jon B. Klein, and Kenneth R. McLeish_x000D_ _x000D_ Index_x000D_