Description
SPRINGER Two-Hybrid Systems 2001 Edition by MACDONALD, PAUL N.
Many, if not all, essential biological processes require selective interactions between proteins. Complex signaling systems require sequential, ordered protein-protein interactions at essentially all levels of the signaling cascade. For example, peptide hormones interact with selective membrane receptor proteins, and autophosphorylation of the receptor then recruits other key regulatory proteins that initiate kinase cascades in which each phosphorylation event requires selective recognition of the protein substrate. The ultimate signaling effect, in many cases, is the regulation of RNA polymerase II-directed transcr- tion in the nucleus, a process that involves numerous, multiprotein complexes important for transcription initiation, elongation, termination, and reinitiation. Defining, characterizing, and understanding the relevance of these protein- protein interactions is an arduous task, but substantial inroads have been made over the past 20 years.The development of more recent methodologies, such as mammalian expression systems, immunopurification schemes, expression cloning strategies, surface plasmon resonance (BiaCore), and nanosequencing technologies, has contributed a wealth of new insights into these complex multiprotein mechanisms and clearly accelerated the discovery process. Arguably, the yeast two-hybrid system has been one of the predominant and most powerful tools in this discovery process. Part I. General MethodsThe Two-Hybrid System: A Personal ViewStanley Fields and Paul L. BartelGrowth and Maintenance of YeastLawrence W. BergmanMedia Formulations for Various Two-Hybrid SystemsMichael Saghbini, Denise Hoekstra, and Jim GautschYeast Two-Hybrid Vectors and StrainsPhilip JamesHigh-Efficiency Transformation of Plasmid DNA into YeastRobin A. Woods and R. Daniel GietzQualitative and Quantitative Assessment of InteractionsMonica M. MontanoStrategies for Rescuing Plasmid DNA from Yeast Two-Hybrid Colonies,Alyson Byrd and Rene St-ArnaudPart II. False PositivesTwo-Hybrid System and False Positives: Approaches to Detection and EliminationIlya G. Serebriiskii and Erica A. GolemisConfirming Yeast Two-Hybrid Protein Interactions Using In Vitro Glutathione-S-Transferase PulldownsDennis M. Kraichely and Paul N. MacDonaldTwo-Hybrid Interactions Confirmed by Coimmunoprecipitation of Epitope-Tagged ClonesLouie NaumovskiPart III. ApplicationsSmad Interactors in Bone Morphogenetic Protein SignalingXiangli Yang and Xu CaoProtein Interactions Important in Eukaryotic Translation InitiationKatsura Asano and Alan G. HinnebuschSteroid Receptor and Ligand-Dependent Interaction with Coactivator ProteinsSergio A. OnateInteraction of Cellular Apoptosis Regulating Proteins with Adenovirus Anti-Apoptosis Protein E1B-19KThirugnana Subramanian and G. ChinnaduraiPart IV. Alternative StrategiesMammalian Two-Hybrid Assays: Analyzing Protein-Protein Interactions in the Transforming Growth Factor-_ Signaling PathwayXin-Hua Feng and Rik DerynckOne-Hybrid Systems for Detecting Protein-DNA InteractionsMary Kate Alexander, Brenda D. Bourns, and Virginia A.ZakianThe Split-Hybrid System: Uncoding Multiprotein Networks and Defining Mutations That Affect Protein InteractionsPhyllis S. Goldman, Anthony J. DeMaggio, Richard H. Goodman, and Merl F. HoekstraThree-Hybrid Screens: Inducible Third-Party SystemsBjoern Sandrock, Franck Tirode, and Jean-Marc EglyThree-Hybrid Screens for RNA-Binding Proteins: Proteins Binding 3' End of Histone mRNAZbigniew Dominski and William F. MarzluffMembrane Recruitment Systems for Analysis of Protein-Protein InteractionsAmi Aronheim