Description
Humana Vaccinia Virus And Poxvirology Methods And Protocols by Stuart Isaacs
A comprehensive collection of cutting-edge methods to study and work with the vaccinia virus and other poxvirus gene transcriptions. These readily reproducible techniques can be used for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; for the investigation of the binding, entry, and movement of the virus in host cells; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxviruses - the latter a critical ability given the important role of vaccinia virus in smallpox vaccination and their potential role as vaccine vectors directed against infectious agents and cancer. Working Safely with Vaccinia Virus: Laboratory Technique and the Role of Vaccinia Vaccination Stuart N. Isaacs Construction and Isolation of Recombinant Vaccinia Virus Using Genetic Markers Maria M. Lorenzo, Inmaculada Galindo, and Rafael Blasco Construction of Recombinant Vaccinia Virus: Cloning into the Thymidine Kinase Locus Chelsea M. Byrd and Dennis E. Hruby Transient and Inducible Expression of Vaccinia/T7 Recombinant Viruses Mohamed Ragaa Mohamed and Edward G. Niles Construction of Recombinant Vaccinia Viruses Using Leporipoxvirus-Catalyzed Recombination and Reactivation of Orthopoxvirus DNA, Xiao-Dan Yao and David H. Evans Construction of cDNA Libraries in Vaccinia Virus Ernest S. Smith, Shuying Shi, and Maurice Zauderer Construction and Isolation of Recombinant MVA Caroline Staib, Ingo Drexler, and Gerd Sutter Growing Poxviruses and Determining Virus Titer Girish J. Kotwal and Melissa-Rose Abrahams Rapid Preparation of Vaccinia Virus DNA Template for Analysis and Cloning by PCR Rachel L. Roper Orthopoxvirus Diagnostics Hermann Meyer, Inger K. Damon, and Joseph J. Esposito An In Vitro Transcription System for Studying Vaccinia Virus Early Genes Steven S. Broyles and Marcia Kremer An In Vitro Transcription System for Studying Vaccinia Virus Late Genes Cynthia F. Wright Studying Vaccinia Virus RNA Processing In Vitro Paul D. Gershon Methods for Analysis of Poxvirus DNA Replication Paula Traktman and Kathleen Boyle Studying the Binding and Entry of the Intracellular and Extracellular Enveloped Forms of Vaccinia Virus Mansun Law and Geoffrey L. Smith Pox, Dyes, and Videotape: Making Movies of GFP-Labeled Vaccinia Virus Brian M. Ward Interaction Analysis of Viral Cytokine-Binding Proteins Using Surface Plasmon Resonance Bruce T. Seet and Grant McFadden Monitoring of Human Immunological Responses to Vaccinia Virus Richard Harrop, Matthew G. Ryan, Hana Golding, Irina Redchenko, and Miles W. Carroll Vaccinia Virus as a Tool for Immunologic Studies Nia Tatsis, Gomathinayagam Sinnathamby, and Laurence C. Eisenlohr Mouse Models for Studying Orthopoxvirus Respiratory Infections Jill Schriewer, R. Mark L. Buller, and Gelita Owens Viral Glycoprotein-Mediated Cell Fusion Assays Using Vaccinia Virus Vectors Katharine N. Bossart and Christopher C. Broder Use of Dual-Recombinant Vaccinia Virus Vectors to Assay Viral Glycoprotein-Mediated Fusion with Transfection-Resistant Primary Cell Targets Yanjie Yi, Anjali Singh, Joanne Cutilli, and Ronald G. Collman Poxvirus Bioinformatics Chris Upton Preparation and Use of Molluscum Contagiosum Virus from Human Tissue Biopsy Specimens Nadja V. Melquiot and Joachim J. Bugert Index