Description
SPRINGER Vascular Disease 1999 Edition by BAKER, ANDREW H. (ED.)
Molecular biology has revolutionized research into vascular disease. Over the past 20 years molecular techniques have enabled us to both elucidate - lecular mechanisms in vascular disease and identify appropriate therapies. The vast explosion in technical knowledge and the array of protocols that become more advanced and intricate by the day lead us into new and exciting areas of research that were previously unobtainable. Vascular Disease: Molecular Biology and Gene Transfer Protocols - scribes today's most powerful molecular methods for the investigation of the pathogenesis of vascular disease. The protocols are highly detailed, allowing beginners who have little experience in either vascular biology or molecular biology to embark on new molecular projects. This book is also suited to more experienced molecular biologists who wish to grasp new methods for stu- ing the involvement of genes in normal vascular physiology and in diseased states. It is well established that cardiovascular disease progression has a s- stantial genetic influence. Part I describes three methods that have been used successfully to identify specific mutations in candidate genes involved in c- diovascular disorders. These mutations include both single-stranded conf- mational polymorphism analysis and heteroduplex detection methods. In addition, technology to map new genes to specific regions of chromosomes by high-resolution mapping is described. Part I. Genetics in Vascular DiseaseDetection of Mutations and DNA Polymorphisms in Genes Involved in Cardiovascular Diseases by Polymerase Chain Reaction-Single-Strand Conformation Polymorphism AnalysisShu Ye and Adriano M. HenneyAnalysis of Genetic Variants in Cardiovascular Risk Genes by Heteroduplex AnalysisAna Cenarro, Fernando Civeira, and Miguel PocoviRadiation Hybrid (RH) Mapping of Human Smooth Muscle-Restricted GenesJoseph M. Miano, Emilio Garcia, and Ralf KrahePart II. Isolation of Genes Expressed in Vascular TissueEfficient Extraction of RNA from Vascular TissueCatherine F. Townsend, Christopher M. H. Newman, and Sheila E. FrancisPreparation of cDNA Libraries from Vascular CellsMark E. Lieb and Mark B. TaubmanScreening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular CellsCsilla Csortos, Virginie Lazar, and Joe G. N. GarciaCloning Full-Length cDNAs from Vascular Tissues and Cells by Rapid Amplification of cDNA Ends (RACE) and RT-PCRRong-Fong ShenUse of cDNA Representational Difference Analysis to Identify Disease-Specific Genes in Human Atherosclerotic PlaquesKerry Tyson and Catherine ShanahanThe Use of Differential mRNA Display (DDRT-PCR) to Identify Genes Differentially Expressed in Normal and Diseased Vascular CellsPaul J. AdamIdentification of Novel Protein Kinases in Vascular CellsIan Zachary, Spiros Servos, and Barbara HerrenPart III. Methods for Mapping Transcriptional Start Sites and Measurement of Promoter Activity in Vascular CellsPrimer Extension Analysis to Map Transcription Start Sites of Vascular GenesYutaka Kitami and Kunio HiwadaUse of Liposome-Mediated DNA Transfection to Determine Promoter Activity in Smooth Muscle CellsRosalind P. FabunmiNuclear Run-On Assayto Study Gene Transcription in Vascular CellsUlrich Laufs and James K. LiaoElectromobility Shift Analysis (EMSA) Applied to the Study of NF-kappa B Binding Interactions in Vascular Smooth Muscle CellsTodd BourcierPart IV. Molecular Analysis of mRNA Expression in Vascular CellsMeasurement of Gene Expression in the Vascular Wall by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) AnalysisJoachim FruebisNorthern Blot Analysis to Quantify Gene Expression in Vascular DiseasesMingyi Chen and Tatsuya SawamuraLocalization of Gene Expression in Human Atherosclerotic Lesions by In Situ HybridizationLee D. K. Buttery and Julia M. PolakRNase Protection Assays for Quantification of Gene Expression in Vascular TissueNengyu Yang, Shuli Wang, and James E. FaberPart V. Molecular Methods to Study Apoptosis and Phenotypic Changes in Vascular Cells and TissueDetection of Apoptosis in Atherosclerosis and Restenosis by Terminal dUTP Nick End Labeling (TUNEL)Mark Kockx, Johannes Muhring, and Michiel KnaapenIn Vitro Detection of Apoptosis in Isolated Vascular CellsShiu-Wan Chan and Martin R. BennettMolecular Assessment of the Phenotypic Changes Associated with Smooth Muscle Cells Using Two-Dimension Electrophoresis and Microsequence AnalysisRachel Johnatty, Giulio Gabbiani, and Pascal NeuvillePart VI. In Vitro Methods to Express Foreign Genes in Vascular CellsSimple Methods for Preparing Recombinant Adenoviruses for High-Efficiency Transduction of Vascular CellsStuart A. Nicklin and Andrew H. BakerGeneration of Recombinant Adeno-Associated Viruses for Delivery of Genes into Vascular CellsCarmel M. LynchHemagglutinating Virus of Japan Liposome-Mediated Gene Delivery to Vascular CellsYoshikazu Yonemitsu and Yasufumi KanedaHigh-Efficien