Description
Taylor & Francis Virus Separation and Purification Methods 1993 Edition by Alfred Polson
This reference presents many contributions made by Dr Alfred Polson during his 41 years of research into the physicochemical properties of plant and animal viruses - detailing his timesaving approaches to the characterization, extraction, separation, concentration and purification of viruses, proteins, antibodies and biopolymers.Describing successful laboratory techniques featuring the Beckman preparative centrifuge, this reference: examines the electro-extraction process for isolating the maximum amount of viruses from infected plant material analyzes zone electrophoresis in sugar concentration gradients as a powerful tool for purifying entero-, insect born and insect viruses contains material on the use of a reorienting gradient centrifuge rotor to separate components of the haemolymph of the mollusc Turbo sarmaticus as a model for virus separation explains the construction of a modified thin-layer ultracentrifuge rotor for concentrating viruses into suspension rather than pellets elucidates the use of inserts in ultracentrifuge tubes in order to decrease the time of centrifuging and introduces a method for determining pure viruse suspension densities.This resource is intended for microbiologists, virologists, biochemists, molecular and cell biologists, immunologists, biochemical and bioprocess engineers, chemical engineers and graduate students in these disciplines. Table of contents :- Methodology Purification of Viruses by Electrophoresis in Sucrose Concentration Gradients A Theory for the Displacement of Proteins and Viruses with Polyethylene Glycol Isolation of Viruses by Electro-Extraction of Infected Plants Preparative Immunoabsorption Electrophoresis An Insert for the Reorienting Gradient Rotor for Virus Extraction from Infected Plants The Sedimenting-Droplet Procedure for Measuring Fluid Densities Determination of the Number of Fab, Pieces Which React with High molecular Weight Antigens Tube Inserts as Aids in Preparative Ultracentrifugation Bead centrifuge Tube Inserts: A Semi-Quantitative Theory Purification of Viruses by Centrifugation in Gradients of Inert Substances Separation and Purification of SpecificViruses A Rapid Method for the Purification of the MEF, Strain of Poliomyelitis Virus Separation of Small Infective Components of MEF, Poliomyelitis and Horsesickness Viruses by Migration into Agar Gels Isolation of the Small Infective Particle in Adapted MEF, Poliomyelitis Studies on Poliomyelitis Virus Concentration and Purification of the Virus Investigations of Poliomyelitis Antigens Specific Precipitin Reactions of the Virus of Poliomyelitis in Gels Purification and Aggregation of Influenza Virus by Precipitation with Polyethylene Glycol Isolation of a Cytoplasmic Polyhedrosis Virus by Physical and Immunological Techniques Purification of Filamentous Plant Viruses by Thin Layer Centrifugation Electro Extraction of Viruses from Infected Plant Purification of Potato Leaf Roll Virus Purification of PVY' and Its Soluble Antigen by Physicochemical Means