Description
Springer Human T-Lymphotropic Viruses Methods and Protocols 1st Editon 2017 Hardbound by Claudio Casoli
While HIV-1 continues to be well-researched, this detailed volume draws attention to other members of the Retrovirus family, namely the Human T-lymphotropic Viruses (HTLVs), featuring the most updated technical information about HTLV determination and the methods to investigate their interaction with the host immune system and interfering pathogens. The contents include essential aspects of epidemiology and virus transmission, novel and robust methodologies for studying the effects of trans-activating regulatory HTLVs’ proteins, the latest techniques for genotyping and gene expression analysis, as well as cellular phenotype and dynamics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Human T-Lymphotropic Viruses: Methods and Protocols serves as an ideal guide to an area of study that is very much worthy of further research. Serological and Molecular Methods to Study Epidemiological Aspects of Human T-Cell Lymphotropic Virus Type 1 Infection.- Molecular Epidemiology Database for Sequence Management and Data Mining.- Reporter Systems to Study HTLV-1 Transmission.- Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Infection Using Co-Culture with Jurkat LTR-Luciferase or Jurkat LTR-GFP Reporter Cells.- Isolation of Exosomes from HTLV-Infected Cells.- A Luciferase Functional Quantitative Assay for Measuring NF-ĸB Promoter Transactivation Mediated by HTLV-1 and HTLV-2 Tax Proteins.- Generation of a Tet-On Expression System to Study Transactivation Ability of Tax-2.- EGF Uptake and Degradation Assay to Determine the Effect of HTLV Regulatory Proteins on the ESCRT-Dependent MVB Pathway.- Methods for Identifying and Examining HTLV-1 HBZ Post-Translational Modifications.- High-Throughput Mapping and Clonal Quantification of Retroviral Integration Sites.- STR Profiling of HTLV-1-Infected Cell Lines.- Expression of HTLV-1 Genes in T-cells Using RNA Electroporation.- Quantification of Cell Turnover in the Bovine Leukemia Virus Model.- Analysis of NK Cell Function and Receptor Expression During HTLV-1 and HTLV-2 Infection.- Overview of Targeted Therapies for Adult T-Cell Leukemia/Lymphoma.