Description
Wiley India Introduction To Biotechnology A Genetic Manipulation Perspective by Rup Lal
An Introduction to Biotechnology: A Genetic Manipulation Perspective contains practical exercises, and has a comprehensive coverage on the subjects of biotechnology and gene manipulation for the students’ understanding of the basic concepts. This manual contains, in particular, the techniques, protocols and practical approaches that are being used routinely in molecular biology laboratories. This book is assembled together with an aim to enlighten the readers with the complex molecular biology protocols in a simple and straightforward manner.
About the Author
Prof. Rup Lal is also the Dean (Examination) at the same university. His primary research interests include elucidating microbial diversity using culture dependent and culture independent (metagenomics) approaches from stressed environments. He has characterized nearly 40 bacterial species, 12 bacterial genomes and three metagenomes from stressed niches. Apart from this, his group has explored the genetics and biochemistry of hexachlorocyclohexane (HCH) degradation, especially using sphingomonads isolated from the HCH dump site.
TABLE OF CONTENTS
BOOK REVIEW
Introduction to Biotechnology
Introduction to Gene Manipulation
Preparation of Media and Chemicals
Growth and Maintenance of Culture
Isolation of Genomic DNA
Isolation of Plasmid DNA
Isolation of RNA
Visualization of Nucleic Acids and Proteins
Whole Cell Protein Isolation and SDS Page
Quantification of DNA
Primer Designing
Polymerase Chain Reaction (PCR)
Restriction Digestion
Purification of DNA from an Agarose Gel
Ligation
Transformation
Cloning and Cloning Vectors
DNA Blot Hybridization
DNA Sequencing