Description
Springer Vaccine Design Methods and Protocols Volume 2 Vaccines for Veterinary Diseases 1st Editon 2018 Softbound by Sunil Thomas
This text provides a practical guide providing step-by-step protocol to design and develop vaccines. Chapters detail protocols for developing novel vaccines against infectious bacteria, viruses, fungi, and parasites for humans and animals. Volume 2: Vaccines for Veterinary Diseases includes vaccines for farm animals and fishes, vaccine vectors and production, vaccine delivery systems, vaccine bioinformatics, vaccine regulation and intellectual property. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Vaccine Design: Methods and Protocols, Volume 2: Vaccines for Veterinary Diseases aims to ensure successful results in the further study of this vital field. Challenges in Veterinary Vaccine Development and Immunization.- Development of Mycoplasma hyopneumoniae Recombinant Vaccines.- Computational Prediction of Immunodominant Epitopes on Outer Membrane Protein (Omp) H of Pasteurella Multocida towards Designing of a Peptide Vaccine.- DNA Vaccines against Maedi-Visna Virus.- Detection of Avian Antigen-Specific T cells Induced by Viral Vaccines.- Generation of Newcastle Disease Virus (NDV) Recombinants Expressing the Infectious Laryngotracheitis Virus (ILTV) Glycoprotein gB or gD as Dual Vaccines.- A Reverse Genetics Approach for the Design of Methyltransferase-Defective Live Attenuated Avian Metapneumovirus Vaccines.- Development of Fasciola Vaccine in an Animal Model.- Development of Experimental Vaccines against Liver Flukes.- Towards a Preventive Strategy for Toxoplasmosis: Current Trends, Challenges, and Future Perspectives for Vaccine Development.- DNA Vaccination in Chickens.- Selection of Vaccine Candidates for Fish Pasteurellosis Using a Reverse Vaccinology and an In Vitro Screening Approach.- Development of Vaccines against Nocardia in Fishes.- Design of an Immersion Vaccine against Aeromonad Septicaemia in Perch (Perca fluviatilis L.)..- Prokaryotic Production of Virus-Like Particle Vaccine of Betanodavirus.- Design and Construction of Shrimp Antiviral DNA Vaccines Expressing Long and Short Hairpins for Protection by RNA Interference.- Developing Anti-Tick Vaccines.- Host Immunization with Recombinant Proteins to Screen Antigens for Tick Control.- Vaccinomics Approach to Tick Vaccine Development.- Development of CpG ODN Based Vaccine Adjuvant Formulations.- Assembly and Assessment of DNA Scaffolded Vaccines.- Alphavirus-based Vaccines.- Vaccine Design: Replication-Defective Adenovirus Vectors.- Generation of Lymphocytic Choriomeningitis Virus based Vaccine Vectors.- Production of Japanese Encephalitis Virus-like Particles Using Insect Cell Expression Systems.- ;Subunit Protein Vaccine Delivery System for Tuberculosis Based on Hepatitis B Virus Core VLP (HBc-VLP) Particles.- Formulation Studies during Preclinical Development of Influenza Hemagglutinin and Virus-like Particle Vaccine Candidates.- Strategies for Vaccine Design using Phage Display-Derived Peptides.- Production of Well-Characterized Virus-Like Particles in an Escherichia coli based Expression Platform for Preclinical Vaccine Assessments.- Laboratory Scale Production of Recombinant Haa86 Tick Protein in Pichia pastoris and in Escherichia coli System.- E. Coli.- Immunoproteomic Approach for Screening Vaccine Candidates from Bacterial Outer Membrane Proteins.- Construction and Immunogenicity Testing of Whole Recombinant Yeast-Based T cell Vaccines.- Oral Rabies Vaccine Design For Expression in Plants.- Purification of Virus-like Particles (VLPs) from Plants.- Transient Expression of Viral Proteins in Plants using Agrobacterium tumefaciens.- A Plant-Based Transient Expression System for the Rapid Production of Malaria Vaccine Candidates.- Recombinant Botulinum Toxoids: A Practical Guide for Production.- Practical Approaches to Forced Degradation Studies of Vaccines.- Preparation of Multifunctional Liposomes as a Stable Vaccine Delivery-Adjuvant System by Procedure of Emulsification-Lyophilization.- Preparation of the Multifunctional Liposome-Containing Microneedle Arrays as an Oral Cavity Mucosal Vaccine Adjuvant-Delivery System.- Preparation and Characterization of PLGA Encapsulated Protective Antigen Domain 4 Nanoformulation.- Attenuated Salmonella sp. as a DNA delivery system for T. cruzi antigens.- Poly-e-Caprolactone/Chitosan and Chitosan Particles: Two Recombinant Antigen Delivery Systems for Intranasal Vaccination.- Micro-Fractional Epidermal Powder Delivery for Skin Vaccination.- A Bioinformatics Method for the Design of Live Attenua